Active Recombinant Human USP9X, GST-tagged

• Cat.No.: USP9X-184H
• Product Overview: Recombinant human USP9X (amino acid residues 1554-1995), fused with N-terminal GST, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1554-1995 a.a.
• Description: USP9X acts by removing monoubiquitin from SMAD4, thereby permitting its association with phospho-SMAD2 and subsequent activation of TGF-β/SMAD-responsive gene targets. USP9X has also been shown to control AMP-activated protein kinase (AMPK)-related kinase activity through direct removal of non-canonical K29 and/or K33-linked ubiquitin chains (Sacco et al., 2010). USP9X can also stabilise levels of myeloid cell leukemia sequence 1 protein (MCL1) and thereby promotes cell survival. USP9X binds MCL1 and removes the Lys 48-linked polyubiquitin chains that normally mark MCL1 for proteasomal degradation.
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-USP9x was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-USP9x was compared confirming the deubiquitylating activity of GST-USP9x.
• Molecular Mass: ~79 kDa
• Purity: >78% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: USP9X ubiquitin specific peptidase 9, X-linked [ Homo sapiens ]
• Official Symbol: USP9X
• Synonyms: USP9X; ubiquitin specific peptidase 9, X-linked; ubiquitin specific peptidase 9, X linked (fat facets like, Drosophila) , ubiquitin specific protease 9, X chromosome (fat facets like Drosophila) , ubiquitin specific protease 9, X linked (fat facets like, Drosophila); probable ubiquitin carboxyl-terminal hydrolase FAF-X; DFFRX; FAF; hFAM; fat facets in mammals; ubiquitin thioesterase FAF-X; deubiquitinating enzyme FAF-X; ubiquitin thiolesterase FAF-X; fat facets protein related, X-linked; fat facets protein-related, X-linked; Drosophila fat facets related, X-linked; ubiquitin-specific protease 9, X chromosome; ubiquitin-specific processing protease FAF-X; ubiquitin-specific-processing protease FAF-X; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); FAM
• Gene ID: 8239
• mRNA Refseq: NM_001039590
• Protein Refseq: NP_001034679
• MIM: 300072
• UniProt ID: Q93008
• Chromosome Location: Xp11.4
• Function: co-SMAD binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity