Active Recombinant Human ULK1, GST-tagged
Cat.No. : | ULK1-1507H |
Product Overview : | Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
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Species : | Human |
Source : | Sf9 Cells |
Tag : | GST |
Protein Length : | 1-649 aa |
Description : | ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner. |
Form : | Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. |
Bio-activity : | 50 nmol/min/mg |
Molecular Mass : | ~125 kDa |
Purity : | >70% |
Applications : | Kinase Assay, Western Blot |
Storage : | Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles. |
Concentration : | 0.1μg/μl |
Gene Name | ULK1 unc-51-like kinase 1 (C. elegans) [ Homo sapiens ] |
Official Symbol | ULK1 |
Synonyms | ULK1; unc-51-like kinase 1 (C. elegans); unc 51 (C. elegans) like kinase 1; serine/threonine-protein kinase ULK1; ATG1; ATG1 autophagy related 1 homolog (S. cerevisiae); ATG1A; ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog; UNC51; hATG1; Unc51.1; FLJ38455; FLJ46475; |
Gene ID | 8408 |
mRNA Refseq | NM_003565 |
Protein Refseq | NP_003556 |
MIM | 603168 |
UniProt ID | O75385 |
Chromosome Location | 12q24.3 |
Pathway | Regulation of autophagy, organism-specific biosystem; Regulation of autophagy, conserved biosystem; Senescence and Autophagy, organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem; mTOR signaling pathway, conserved biosystem; |
Function | ATP binding; nucleotide binding; protein binding; protein complex binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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