Active Recombinant Human UGT1A1 Protein

• Cat.No.: UGT1A1-97H
• Product Overview: Recombinant human UGT1A1 protein without tag was expressed in insect cells.

Specification

• Description: This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.
• Source: Insect cells
• Species: Human
• Bio-activity: UGT Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM Tris-HCl (pH 7.5) containing 5 mM magnesium chloride and 2 mM UDPGA. Alamethicin (10 μg/ml final concentration) is added to kinetics tests only)
  Substrate: 17β-estradiol (3-glucuronide formation)
  Linear with time up to: 20 min(Determined at 10 μM substrate, using a protein concentration of 250 μg/ml)
  Linear with protein up to: 500 μg/ml(Determined at 10 μM substrate, using an incubation time of 5 min)
  Vmax: 797 pmol/min/mg protein
  S50: 10 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: Protein Concentration: 5 mg/ml
• Storage Buffer: 25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA

Gene Information

• Gene Name: UGT1A1 UDP glucuronosyltransferase family 1 member A1 [ Homo sapiens (human) ]
• Official Symbol: UGT1A1
• Synonyms: UGT1A1; UDP glucuronosyltransferase family 1 member A1; GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1; UDP-glucuronosyltransferase 1A1; UDP glucuronosyltransferase 1 family, polypeptide A1; UDP glycosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A; UGT-1A; UGT1*1; UGT1-01; UGT1.1; bilirubin UDP-glucuronosyltranserase; bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; bilirubin-specific UDPGT isozyme 1
• Gene ID: 54658
• mRNA Refseq: NM_000463
• Protein Refseq: NP_000454
• MIM: 191740
• UniProt ID: P22309