Active Recombinant Human UBE2O Protein, GST-tagged

• Cat.No.: UBE2O-651H
• Product Overview: Recombinant Human UBE2O fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Description: UBE2O or ubiquitin-conjugating enzyme E2O is a part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin. HIV-1 Tat has been identified to have a physical interaction with ubiquitin-conjugating enzyme E2O (UBE2O) in human HEK293 and Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses.
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
• Bio-activity: 25 nmol/min/mg
• Molecular Mass: 106 kDa
• Purity: >95%
• Applications: Ubiquitin Enzyme Assay, Western Blot
• Stability: 1 year at - 70 centigrade from the date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ml

Gene Information

• Gene Name: UBE2O ubiquitin-conjugating enzyme E2O [ Homo sapiens ]
• Official Symbol: UBE2O
• Synonyms: UBE2O; ubiquitin-conjugating enzyme E2O; ubiquitin-conjugating enzyme E2 O; E2 230K; ubiquitin-protein ligase O; ubiquitin carrier protein O; ubiquitin-conjugating enzyme E2-230K; ubiquitin-conjugating enzyme E2 of 230 kDa; E2-230K; FLJ12878; KIAA1734
• Gene ID: 63893
• mRNA Refseq: NM_022066
• Protein Refseq: NP_071349
• UniProt ID: Q9C0C9