Active Recombinant Human TXN protein, His-tagged

• Cat.No.: TXN-2585H
• Product Overview: Active Recombinant Human Thioredoxin/SASP/TXN Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val2-Val105) of human Thioredoxin (Accession #NP_003320.2) fused with a 6×His tag at the C-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human TXN Protein at 1 μg/mL (100 μL/well) can bind TXN Rabbit mAb with a linear range of 0.976-5.3 ng/mL.
  2.Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >9 A650/min/mg.
• Molecular Mass: 13 kDa
• Protein length: Val2-Val105
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: TXN thioredoxin [ Homo sapiens ]
• Official Symbol: TXN
• Synonyms: TXN; thioredoxin; TRX; ADF; SASP; TXN delta 3; ATL-derived factor; thioredoxin delta 3; surface-associated sulphydryl protein; TRDX; TRX1; MGC61975; DKFZp686B1993
• Gene ID: 7295
• mRNA Refseq: NM_001244938
• Protein Refseq: NP_001231867
• MIM: 187700
• UniProt ID: P10599