Active Recombinant Human Tumor Necrosis Factor Receptor Superfamily, Member 14 (Herpesvirus Entry Mediator)

• Cat.No.: TNFRSF14-5144H
• Product Overview: Recombinant human TNFRSF14 was expressed in Hi-5 Insect cells and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: HVEM belongs to the TNF Receptor superfamily of transmembrane proteins and plays a role in the activation of T-cells and other lymphocytes. It is expressed in various cells and tissues including spleen, thymus, lung, macrophages, and T-cells. HVEM activation induces a signaling cascade which results in induction of transcription factors NF-kappaB and AP-1. LIGHT (TNFSF14) and TNF-beta (TNFSF1) function as the ligands for HVEM, which can also bind specifically to herpes simplex virus glycoprotein D. Soluble HVEM can act as a "receptor decoy" resulting in inhibition of the activity of the HVEM ligands, LIGHT and TNF-beta.
• Form: Supplied as a lyophilized powder
• Purity: >98% pure by SDS PAGE and HPLC
• Sequence: Region of HVEM protein corresponding to amino acids LPSCKEDEYP VGSECCPKCS PGYRVKEACG ELTGTVCEPC PPGTYIAHLN GLSKCLQCQM CDPAMGLRAS RNCSRTENAV CGCSPGHFCI VQDGDHCAAC RAYATSSPGQ RVQKGGTESQ DTLCQNCPPG TFSPNGTLEE CQHQTKRSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRT
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Bioactivity: Determined by its ability to neutralize 0.25 ng/ml of hTNFbeta induced cytotoxicity on Mouse L929 cells. The expected ED50 for this effect is 1.3-1.9 ug/ml of HVEM-Fc.
• Applications: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions
• Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: TNFRSF14 tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) [ Homo sapiens ]
• Official Symbol: TNFRSF14
• Synonyms: TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR; tumor necrosis factor receptor superfamily member 14; CD40-like protein; OTTHUMP00000000866; OTTHUMP00000204095; OTTHUMP00000204096; OTTHUMP00000204097; herpesvirus entry mediator A; herpes virus entry mediator A; tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
• Gene ID: 8764
• mRNA Refseq: NM_003820
• Protein Refseq: NP_003811
• MIM: 602746
• UniProt ID: Q92956
• Chromosome Location: 1p36.3-p36.2
• Pathway: Adaptive Immunity Signaling; Costimulation by the CD28 family; Cytokine-cytokine receptor interaction; Cytokine-cytokine receptor interaction; Herpes simplex infection; Immune System
• Function: protein binding; receptor activity; tumor necrosis factor receptor activity