Active Recombinant Human Tryptase Gamma 1, His-tagged

• Cat.No.: TPSG1-510H
• Product Overview: Recombinant Human TPSG1protein was expressed in Murine myeloma cell line with a C-terminal 10-Histag. Arg20-Arg281.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 20-281 a.a.
• Description: Human TPSG1 issynthesized as a 321 amino acid preproenzyme with a C-terminal transmembraneanchor. The proenzyme can be cleaved by trypsin to form the active enzyme. Asmediators of inflammatory and allergic response, mast cells are foundthroughout the body concentrated near blood vessels in connective tissue andthe mucous membranes of the respiratory and gastrointestinal tract.
• Form: Supplied as a 0.2 μmfiltered solution in Tris, CaCl2, NaCl, Brij-35 and Glycerol.
• N-terminalSequence: Arg20
• Molecular Weight: 29 kDa
• Activity: Measuredby its ability to cleave the fluorogenic peptide substrate,Mca-RPKPVE-Nval-WRK (Dnp)-NH2, The specific activity is > 70pmoles/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 36 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months fromdate of receipt, -70°C as supplied. 3 months, -70°C under sterile conditionsafter opening.

Gene Information

• Gene Name: TPSG1 tryptase gamma 1 [ Homosapiens ]
• Official Symbol: TPSG1
• Synonyms: TPSG1; tryptasegamma 1; TMT; trpA; PRSS31; tryptase gamma; gamma I; gamma II; lung tryptase;skin tryptase; tryptase gamma I; tryptase gamma II; mast cell tryptase;pituitary tryptase; serine protease 31; mast cell protease II; transmembranetryptase;EC 3.4.21.59; EC=3.4.21.-; OTTHUMP00000115529
• Gene ID: 25823
• mRNA Refseq: NM_012467
• Protein Refseq: NP_036599
• MIM: 609341
• UniProt ID: Q9NRR2
• Chromosome Location: 16p13.3
• Function: peptidase activity; serine-typeendopeptidase activity; serine-type peptidase activity