Active Recombinant Human TNFSF4, His-tagged

• Cat.No.: TNFSF4-272H
• Product Overview: Recombinant Human TNFSF4 /OX40L Protein (rh TNFSF4 / OX40L) Gln 51 - Leu 183 (Accession # NP_003317) was produced in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 51-183 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA.ImmobilizedHuman OX40 Ligand, His Tag at 5 μg/mL (100 μl/well) can bind Human OX40, Fc Tag with a linear range of 0.2-5 ng/mL.
• Molecular Mass: The SEC-HPLC analysis indicates that more than 90% OX40L protein is active trimeric form , which has a molecular mass of 75 kDa.Human OX40 Ligand, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 16.9 kDa. The predicted N-terminus is His. The reducing (R) protein migrates as 25-30 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF4 tumor necrosis factor (ligand) superfamily, member 4 [ Homo sapiens ]
• Official Symbol: TNFSF4
• Synonyms: TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tax transcriptionally activated glycoprotein 1, 34kD , TXGP1; tumor necrosis factor ligand superfamily member 4; CD252; gp34; OX 40L; OX40L; CD134 ligand; glycoprotein Gp34; OX40 antigen ligand; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); GP34; OX4OL; TXGP1; CD134L; OX-40L
• Gene ID: 7292
• mRNA Refseq: NM_003326
• Protein Refseq: NP_003317
• MIM: 603594
• UniProt ID: P23510
• Chromosome Location: 1q25
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem
• Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor superfamily binding