Active Recombinant Human TNFSF13 protein, His-Flag-tagged

• Cat.No.: TNFSF13-26H
• Product Overview: Recombinant Human TNFSF13 protein(Gln 111 - Leu 250), fused with His-Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: Gln 111 - Leu 250
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human BCMA, Fc Tag at 2 μg/mL (100 μL/well) can bind Human APRIL Protein, His,Flag Tag with a linear range of 10-39 ng/mL.Immobilized Human APRIL Protein, His,Flag Tag at 1 μg/mL (100 μL/well) can bind Human TACI Protein, Fc Tag with a linear range of 0.8-25 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus, followed by a flag tagThe protein has a calculated MW of 50.2 kDa. The protein migrates as 50-55 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF13 tumor necrosis factor (ligand) superfamily, member 13 [ Homo sapiens ]
• Official Symbol: TNFSF13
• Synonyms: TNFSF13; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; APRIL; CD256; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2; TALL2; ZTNF2; TALL-2; TRDL-1; FLJ57090; UNQ383/PRO715
• Gene ID: 8741
• mRNA Refseq: NM_001198622
• Protein Refseq: NP_001185551
• MIM: 604472
• UniProt ID: O75888