Active Recombinant Human TNFRSF11B

• Cat.No.: TNFRSF11B-212H
• Product Overview: Recombinant Human TNFRSF11B (Accession # AAB53709.1) Glu22-Leu401 was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Protein Length: 22-401 a.a.
• Predicted N Terminal: Glu22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL.The ED50 for this effect is typically 8-24 ng/mL in the presence of 12 ng/mL cross-linked Recombinant Human TRAIL/TNFSF10.
• Molecular Mass: Recombinant Human TNFRSF11B has a calculated MW of 43.5 kDa (monomer). In SDS-PAGE migrates as 55 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TNFRSF11B tumor necrosis factor receptor superfamily, member 11b [ Homo sapiens ]
• Official Symbol: TNFRSF11B
• Synonyms: TNFRSF11B; tumor necrosis factor receptor superfamily, member 11b; OPG, osteoprotegerin; tumor necrosis factor receptor superfamily member 11B; OCIF; TR1; osteoprotegerin; osteoclastogenesis inhibitory factor; OPG; MGC29565
• Gene ID: 4982
• mRNA Refseq: NM_002546
• Protein Refseq: NP_002537
• MIM: 602643
• UniProt ID: O00300
• Chromosome Location: 8q24