Active Recombinant Human TIGIT protein, mFc-tagged

• Cat.No.: TIGIT-108H
• Product Overview: Recombinant Human TIGIT protein(Met 22 - Pro 141), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: Met 22 - Pro 141
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human CD155, Fc Tag at 5 μg/mL (100 μL/well) can bind Human TIGIT, Mouse IgG2a Fc Tag with a linear range of 0.5-4 ng/mL. Serial dilutions of TIGIT Antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, Mouse IgG2a Fc Tag: Biotinylated Human Nectin-2, Fc,Avitag binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 40.1 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TIGIT T cell immunoreceptor with Ig and ITIM domains [ Homo sapiens ]
• Official Symbol: TIGIT
• Synonyms: TIGIT; T cell immunoreceptor with Ig and ITIM domains; V set and immunoglobulin domain containing 9 , V set and transmembrane domain containing 3 , VSIG9, VSTM3; T-cell immunoreceptor with Ig and ITIM domains; DKFZp667A205; FLJ39873; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain containing 9; Washington University cell adhesion molecule; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; WUCAM
• Gene ID: 201633
• mRNA Refseq: NM_173799
• Protein Refseq: NP_776160
• MIM: 612859
• UniProt ID: Q495A1