Active Recombinant Human TGFB1 protein, His-tagged

• Cat.No.: TGFB1-2682H
• Product Overview: Active Recombinant Human Latent TGF-beta 1(C33S) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8×His tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu30-Ser390(C33S)
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2 ng/mL.
  2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL.
• Molecular Mass: 13&42&60kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137