Active Recombinant Human TGFB1 Protein, His & Avi-tagged, Biotinylated

• Cat.No.: TGFB1-363H
• Product Overview: Recombinant Human TGFB1 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Ser 390 (Accession # P01137-1 (C33S)). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag. The protein has a calculated MW of 45.0 kDa. The protein migrates as 14 kDa, 40-48 kDa and 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Leu 30 - Ser 390
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ITGAV&ITGB6, His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human TGFB1 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.5-4 ng/mL (QC tested).
  Immobilized Human ITGAV&ITGB8, His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human TGFB1 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.2-4 ng/mL (Routinely tested).
• Molecular Mass: 45.0 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137