Active Recombinant Human TGFB1 Protein (Ala279-Ser390), GMP Grade

• Cat.No.: TGFB1-02HG
• Product Overview: GMP recombinant human TGF-beta 1 protein (Ala279-Ser390) without tag was expressed in Chinese Hamster Ovary cell line and manufactured and tested under cGMP guidelines.

Specification

• Species: Human
• Source: CHO
• Protein Length: Ala279-Ser390
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/mL.
  The specific activity of recombinant human TGF-beta 1 GMP is >1.0 x 107 units/mg, which is calibrated against the human TGF- beta 1 Standard.
• Molecular Mass: Predicted Molecular Mass: 12.8 kDa (monomer)
  SDS-PAGE: 12 kDa, reducing conditions; 24 kDa, non-reducing conditions
• N-terminal Sequence Analysis: Ala-Leu-Asp-Thr-Asn-Tyr-(Cys)-Phe-Ser-Ser
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137