Active Recombinant Human TFPI2, Animal Free

• Cat.No.: TFPI2-129H
• Product Overview: Recombinant human TFPI-2 domain 1, is a 9.3 kDa protein containing 79 amino acids residues. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant AGV212contains a 6-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities. Animal free product.

Specification

• Description: Recombinant human TFPI-2 domain 1 or AGV 212 is a protease inhibitor peptide generated from the first Kunitz domain of the human Tissue Factor Protein Inhibitor 2 (TFPI-2) protein, after site-directed mutagenesis to increase its activity. It is arranged in a single polypeptide chain that is linked by three disulphide bridges. AGV 212 is quite stable and inhibits trypsin with high efficiency and Ki lower than TFPI-2 one. TFPI-2 has been shown to inhibit Endothelial Cell Matrix (ECM) proteases essential for angiogenesis and metastasis.
• Source: Nicotiana benthamiana
• Species: Human
• Form: Lyophilized powder containing phosphate buffer salts, pH 7.1.
• Bio-activity: The activity of the inhibitor is expressed as the amount of trypsin inhibited per milligram of inhibitor. The ability to prevent the hydrolysis of benzoyl-L-arginine ethyl ester hydrochloride by trypsin is measured by spectrophotometer. One mg protein will inhibit 1-1.5 mg trypsin with activity of approximately 10,000 BAEE units per mg protein.
• Molecular Mass: Recombinant human TFPI-2 domain 1, is a 9.3 kDa protein containing 79 amino acids residues.
• AA Sequence: HHHHHHGAAQEPTGNNAEICLLPLDYGPCKALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIEK VPKV
• Endotoxin: < 0.04="" eu="" ug="" protein="" (lal="">
• Purity: >97% by SDS-PAGE gel
• Applications: Trypsin inhibitor, Western blot, Immunogen
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at –20°C for long term storage. Diluted solutions are less stable than concentrated ones. Repeated freezing and thawing is not recommended.
• Reconstitution: Lyophilized protein should be reconstituted adding 1 ml of sterile water to the vial, which gives a concentration of 1 mg of protease inhibitor per ml. At higher concentration the solubility may be reduced and multimers generated. Soluble in water and in aqueous buffers of low ionic strengths. Repeated freeze-thaw cycles should be avoided. Optimal reconstitution please follow batch Quality Control sheet instructions.
• Tag: Non

Gene Information

• Gene Name: TFPI2 tissue factor pathway inhibitor 2 [ Homo sapiens ]
• Official Symbol: TFPI2
• Synonyms: TFPI2; tissue factor pathway inhibitor 2; PP5; REF1; TFPI 2; placental protein 5; TFPI-2; FLJ21164
• Gene ID: 7980
• mRNA Refseq: NM_006528
• Protein Refseq: NP_006519
• MIM: 600033
• UniProt ID: P48307
• Chromosome Location: 7q
• Function: extracellular matrix structural constituent; peptidase inhibitor activity; serine-type endopeptidase inhibitor activity