Active Recombinant Human TFPI protein, His-tagged

• Cat.No.: TFPI-875H
• Product Overview: Recombinant Human TFPI protein(Asp 29 - Lys 282), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Asp 29 - Lys 282
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate. The IC50 value is <0.50 nM, as measured under the described conditions.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  MG1113, a specific anti–tissue factor pathway inhibitor antibody, rebalances the coagulation system and promotes hemostasis in hemophilia (2020)

Gene Information

• Gene Name: TFPI tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) [ Homo sapiens ]
• Official Symbol: TFPI
• Synonyms: TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); LACI; tissue factor pathway inhibitor; EPI; extrinsic pathway inhibitor; TFI; TFPI1; anti-convertin
• Gene ID: 7035
• mRNA Refseq: NM_001032281
• Protein Refseq: NP_001027452
• MIM: 152310
• UniProt ID: P10646