Active Recombinant Human SULT1E1, His-tagged

• Cat.No.: SULT1E1-132H
• Product Overview: Recombinant Human SULT1E1, fused with an N-terminal 6-His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors.
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and DTT.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is >40 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass: 36 kDaSDS-PAGE: 35 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 ºC as supplied.3 months, -70 ºC under sterile conditions after opening.

Gene Information

• Gene Name: SULT1E1 sulfotransferase family 1E, estrogen-preferring, member 1 [ Homo sapiens (human) ]
• Official Symbol: SULT1E1
• Synonyms: EC 2.8.2.4; EST; EST-1; MGC34459; STE; Sulfotransferase, estrogen-preferring; estrogen sulfotransferase; estrone sulfotransferase; sulfotransferase family 1E, estrogen-preferring, member 1; STE; SULT1E1
• Gene ID: 6783
• mRNA Refseq: NM_005420
• Protein Refseq: NP_005411
• MIM: 600043
• UniProt ID: P49888
• Chromosome Location: 4q13.1
• Pathway: Biological oxidations; Defective GCLC causes Hemolytic anemia due to gamma-glutamylcysteine synthetase deficiency (HAGGSD); Defective OPLAH causes 5-oxoprolinase deficiency (OPLAHD)
• Function: estrone sulfotransferase activity; steroid binding; steroid sulfotransferase activity