Active Recombinant Human ST14, His-tagged

• Cat.No.: ST14-256H
• Product Overview: Recombinant Human ST14(Gly596­Val855) fused with an N­terminal Met and 6­His tag, was expressed in E. coli.

Specification

• Cat. No.: ST14-256H
• Description: The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis.
• Source: E. coli
• Molecular Mass: Predicted molecular mass is 26 kDa; Other fragments of 19 kDa, 17 kDa, 9 kDa and 6 kDa are also observed in the recombinant protein preparation. It migrates as 27 kDa (major), 20 kDa, 17 kDa, 10 kDa, 6.5 kDa, under reducing conditions.
• Predicted N terminal: Val615
• Formulation: Supplied as a 0.2 μm filtered solution in Tris­HCl and Glycerol.
• Purity: >90% as determined by SDS­PAGE
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc­QAR-AMC.
• Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
• Storage: 6 months from date of receipt, ­20 to ­70°C as supplied. Avoid repeated freeze­thaw cycles.

Gene Information

• Gene Name: ST14 suppression of tumorigenicity 14 (colon carcinoma) [ Homo sapiens ]
• Official Symbol: ST14
• Synonyms: ST14; suppression of tumorigenicity 14 (colon carcinoma); PRSS14; suppressor of tumorigenicity 14 protein; epithin; HAI; matriptase; MT SP1; SNC19; TMPRSS14
• Gene ID: 6768
• mRNA Refseq: NM_021978
• Protein Refseq: NP_068813
• MIM: 606797
• UniProt ID: Q9Y5Y6
• Chromosome Location: 11q24-q25
• Function: serine-type endopeptidase activity; serine-type peptidase activity