Active Recombinant Human SRSF1, His-tagged

• Cat.No.: SRSF1-3067H
• Product Overview: Recombinant HumanSRSF1 is expressed inE.Coliwith a His tag at N-terminus.Molecular Weight: 29kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site.
• Reagents Supplied: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT.
• Quality Control: The purified protein is greater than 95% homogeneous based on SDS-PAGE gel analysis.
• Activity: 1 unit equals 1 nanogram of purified protein. 1-5 units are sufficient for a gel mobility shift assay in a 20 µl reaction, 100 units are sufficient for a protein-protein interaction assay and 100-500 units are required for a splicing assay in a 20 μl reaction.
• Application: Recombinant ASF/SF2 protein can be used for: 1) in vitro function studies including pre-mRNA splicing, cross linking and other RNA binding assays; 2) protein-protein interaction assay; and 3) cell growth and proliferation assays.
• Storage Conditions: Store at -80oC.

Gene Information

• Gene Name: SRSF1 serine/arginine-rich splicing factor 1 [ Homo sapiens ]
• Synonyms: SRSF1; serine/arginine-rich splicing factor 1; ASF-1; SR splicing factor 1; alternative-splicing factor 1; pre-mRNA-splicing factor SF2, P33 subunit; splicing factor 2; splicing factor, arginine/serine-rich 1; ASF; SF2; SFRS1; SF2p33; SRp30a; MGC5228; FLJ53078
• Gene ID: 6426
• mRNA Refseq: NM_001078166
• Protein Refseq: NP_001071634
• MIM: 600812
• UniProt ID: Q07955
• Chromosome Location: 17q22
• Pathway: Spliceosome; Gene Expression; Transcription; mRNA Processing
• Function: RNA binding; nucleotide binding; protein binding