Active Recombinant Human SP1, HA & Flag-tagged

• Cat.No.: SP1-2093H
• Product Overview: Recombinant Human SP1, fused to a HA- and a FLAG-tag, was expressed in insect cells (Schneider SL2 cells).

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag&HA
• Description: The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.
• Form: Liquid. In 50mM TRIS-HCl, pH 7.9, containing 420mM KCl, 0.1% NP-40, 12.5mM MgCl2 and 20% glycerol.
• Bio-activity: 0.5BFU/μl (band forming units). 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
• Applications: EMSA promoter characterization, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
• Quality Control Test: Sufficient for performing at least 25 protein-DNA interaction experiments in 10mM HEPES, pH 7.9, 0.1mM EDTA, 8.5% glycerol, 170mM KCl, 1μM ZnSO4, 37.5μg/ml Poly dIdC, 10mM DTT and 1mg/ml BSA, analyzed by gel shift assay.
• Storage: 80oC. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: SP1 Sp1 transcription factor [ Homo sapiens (human) ]
• Official Symbol: SP1
• Synonyms: SP1; Sp1 transcription factor; Sp1 transcription factor; specificity protein 1; TSFP1; Transcription factor Sp1
• Gene ID: 6667
• mRNA Refseq: NM_003109
• Protein Refseq: NP_003100
• MIM: 189906
• UniProt ID: P08047
• Chromosome Location: 12q13.1
• Pathway: Huntington"s disease; TGF-beta signaling pathway
• Function: RNA polymerase II transcription factor activity; double-stranded DNA binding; histone deacetylase binding; metal ion binding; promoter binding; protein C-terminus binding; protein homodimerization activity; transcription activator activity; transcription factor activity; zinc ion binding