Active Recombinant Human SLPI protein, His-tagged

• Cat.No.: SLPI-2550H
• Product Overview: Recombinant Human SLPI (Met1-Ala132) fussed with His tag at C-terminal was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ser 26
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4.1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2 (Catalog # ES002).The IC50 value is <1 Nm.
• Molecular Mass: The secreted recombinant human SLPI consists of 117 amino acids and predicts a molecular mass of 13.1 KDa. The apparent molecular mass of the protein is approximately 15 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: Met1-Ala132
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: SLPI secretory leukocyte peptidase inhibitor [ Homo sapiens ]
• Official Symbol: SLPI
• Synonyms: SLPI; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); antileukoproteinase; ALK1; ALP; BLPI; HUSI; HUSI I; WAP4; WFDC4; HUSI-1; protease inhibitor WAP4; mucus proteinase inhibitor; seminal proteinase inhibitor; WAP four-disulfide core domain protein 4; MPI; HUSI-I
• Gene ID: 6590
• mRNA Refseq: NM_003064
• Protein Refseq: NP_003055
• MIM: 107285
• UniProt ID: P03973
• Chromosome Location: 20pter-p12.3
• Function: endopeptidase inhibitor activity; enzyme binding; protein binding; serine-type endopeptidase inhibitor activity