Active Recombinant Human SIRPG protein, His-Avi-tagged, Biotinylated

• Cat.No.: SIRPG-0727H
• Product Overview: Biotinylated Recombinant Human SIRPG protein(Glu29-Ser364), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human SIRP gamma, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human CD47, Mouse IgG2a Fc Tag with a linear range of 4-31 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 40.7 kDa. The protein migrates as 48-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu29-Ser364
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 29-364 a.a.

Gene Information

• Gene Name: SIRPG signal-regulatory protein gamma [ Homo sapiens ]
• Official Symbol: SIRPG
• Synonyms: SIRPG; signal-regulatory protein gamma; signal regulatory protein beta 2 , SIRPB2; bA77C3.1; CD172g; SIRP B2; SIRPgamma; SIRP-gamma; SIRP beta 2; SIRP-beta-2; CD172g antigen; signal-regulatory protein beta 2; signal-regulatory protein beta-2; CD172 antigen-like family member B; SIRPB2; SIRP-B2; FLJ42230
• Gene ID: 55423
• mRNA Refseq: NM_001039508
• Protein Refseq: NP_001034597
• MIM: 605466
• UniProt ID: Q9P1W8