Active Recombinant Human SIGLEC11, Fc Chimera

• Cat.No.: SIGLEC11-376H
• Product Overview: Recombinant Human SIGLEC11 (Accession # AAK72907) was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Asn17
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to bind biotinylated (Neu5Aca2-8)2-Polyacrylamide in functional ELISA. Angata, T. et al. (2002) J. Biol. Chem. 277(27):24466.Recombinant Human Siglec-11 Fc Chimera immobilized at 2.5 µg/mL (100 µL/well), on a Goat Anti-Human IgG Fc Antibody coated plate can bind biotinylated (Neu5Ac alpha 2-8)2-Polyacrylamide with a linear range of 0.03-2 µg/mL.
• Molecular Mass: Recombinant Human SIGLEC11, Fc Chimera has a calculated MW of 84.4 kDa (monomer). In SDS-PAGE migrates as 110-135 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SIGLEC11 sialic acid binding Ig-like lectin 11 [ Homo sapiens ]
• Official Symbol: SIGLEC11
• Synonyms: SIGLEC11; sialic acid binding Ig-like lectin 11; sialic acid-binding Ig-like lectin 11; siglec-11
• Gene ID: 114132
• mRNA Refseq: NM_001135163
• Protein Refseq: NP_001128635
• MIM: 607157
• UniProt ID: Q96RL6
• Chromosome Location: 19q13.3