Active Recombinant Human SHH, Met-tagged

• Cat.No.: SHH-606H
• Product Overview: Recombinant Human SHH (Accession # NP_000184) Cys24-Gly197 (Cys24Ile-Ile), fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465.The ED50 for this effect is typically 0.1-0.4 µg/mL.
• Molecular Mass: Recombinant Human SHH, Met-tagged has a calculated MW of 20 kDa. In SDS-PAGE migrates as 22 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Protein length: 24-197 a.a.

Gene Information

• Gene Name: SHH sonic hedgehog [ Homo sapiens ]
• Official Symbol: SHH
• Synonyms: SHH; sonic hedgehog; HLP3, HPE3, sonic hedgehog (Drosophila) homolog , sonic hedgehog homolog (Drosophila); sonic hedgehog protein; HHG1; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; HLP3; HPE3
• Gene ID: 6469
• mRNA Refseq: NM_000193
• Protein Refseq: NP_000184
• MIM: 600725
• UniProt ID: Q15465
• Chromosome Location: 7q36