Active Recombinant Human SERPINE1, His-tagged

• Cat.No.: SERPINE1-535H
• Product Overview: Recombinant human Plasminogen activator inhibitor-1, fused to His tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 45 kDa (400 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 24-402 a.a.
• Description: Plasminogen activator inhibitor-1(PAI-1), a member of the serine protease inhibitor(serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue.
• Form: Liquid. In 150mM Na2HPO4 (pH 6.6) containing 0.5 mM NaCl, 2 mM Glutathione, 0.01% Tween-80.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Bio-activity: The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

Gene Information

• Gene Name: SERPINE1 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 [ Homo sapiens ]
• Synonyms: Plasminogen activator inhibitor 1; Endothelial plasminogen activator inhibitor; PAIE; Clade E; PLANH1; SERPINE 1; SERPINE1; PAI; PAI-1; PAI1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; plasminogen activator inhibitor-1; plasminogen activator inhibitor, type I; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Endothelial plasminogen activator inhibitor
• Gene ID: 5054
• mRNA Refseq: NM_000602
• Protein Refseq: NP_000593
• MIM: 173360
• UniProt ID: P05121
• Chromosome Location: 7q21.3-q22
• Pathway: Complement and coagulation cascades; Hemostasis
• Function: peptidase inhibitor activity; protease binding; protein binding; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity;serine-type endopeptidase inhibitor activity