Active Recombinant Human SEMA5B, Fc Chimera
Cat.No. : | SEMA5B-652H |
Product Overview : | Recombinant Human SEMA5B (Accession # NP_001026872) was produced in Chinese Hamster Ovary cell line, CHO-derived. |
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Source : | CHO cells |
Species : | Human |
Tag : | Fc |
Predicted N Terminal : | Val78 |
Form : | Lyophilized from a 0.2 µm filtered solution in PBS. |
Bio-activity : | Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells.When 5 x 10^4 cells/well are added to Recombinant Human Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is typically 0.7 - 2.8 µg/mL. |
Molecular Mass : | Recombinant Human SEMA5B, Fc Chimera has a calculated MW of 110.3 kDa (monomer). In SDS-PAGE migrates as 130-140 kDa, reducing conditions. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Gene Name | SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B [ Homo sapiens ] |
Official Symbol | SEMA5B |
Synonyms | SemG; SEMAG |
Gene ID | 54437 |
mRNA Refseq | NM_001256348.1 |
Protein Refseq | NP_001243277.1 |
MIM | 609298 |
UniProt ID | Q9P283 |
Chromosome Location | 3q21.1 |
Not For Human Consumption!
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