Active Recombinant Human S100A8 (Met1-Glu93) and S100A9 (Thr2-Pro114) Heterodimer Protein

• Cat.No.: S100A8/S100A9-13H
• Product Overview: Recombinant human S100A8 (Met1-Glu93) and S100A9 (Thr2-Pro114) Heterodimer protein without tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Form: Noncovalently-linked heterodimer
• Bio-activity: Measured by its ability to induce IL-6 secretion by A375 human melanoma cells. The ED50 for this effect is 1.5-6 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 11 kDa (S100A8) & 13 kDa (S100A9)
  SDS-PAGE: 9 kDa (S100A8) & 13 kDa (S100A9), reducing conditions
• Protein length: Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9)
• N-terminal Sequence Analysis: Met1 (S100A8) & Thr2 (S100A9)
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
• Reconstitution: Reconstitute at 500 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: S100A8 S100 calcium binding protein A8 [ Homo sapiens (human) ]
• Official Symbol: S100A8
• Synonyms: S100A8; S100 calcium binding protein A8; CAGA, CFAG, S100 calcium binding protein A8 (calgranulin A), S100 calcium binding protein A8 (calgranulin A); protein S100-A8; 60B8AG; CGLA; MRP8; P8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A); MIF; NIF; CAGA; CFAG; L1Ag; CP-10; MA387
• Gene ID: 6279
• mRNA Refseq: NM_002964
• Protein Refseq: NP_002955
• MIM: 123885
• UniProt ID: P05109