Active Recombinant Human RSPO2 Protein, Fc-tagged

• Cat.No.: RSPO2-480H
• Product Overview: Recombinant human RSPO2 (Q6UXX9-1) N-teminal fragment (Met 1-Gly 205, 186 Leu/Pro) was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-205 a.a.
• Predicted N Terminal: Gln 22
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to induce activation of ?catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.The ED50 for this effect is typically 10-60 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
• Molecular Mass: The secreted recombinant human RSPO2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 425 amino acids and has a predicted molecular mass of 48.3 kDa. ?The apparent molecular mass of rh RSPO2/Fc monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: RSPO2 R-spondin 2 [ Homo sapiens ]
• Official Symbol: RSPO2
• Synonyms: RSPO2; R-spondin 2; R spondin 2 homolog (Xenopus laevis); R-spondin-2; MGC35555; R-spondin 2 homolog; roof plate-specific spondin-2; CRISTIN2; MGC43342
• Gene ID: 340419
• mRNA Refseq: NM_178565
• Protein Refseq: NP_848660
• MIM: 610575
• UniProt ID: Q6UXX9
• Chromosome Location: 8q23.1