Recombinant Active Human RSPO1 Protein, His-tagged(C-ter)

• Cat.No.: RSPO1-278H
• Product Overview: Recombinant Active Human RSPO1 Protein with His tag (C-ter) was expressed in HEK293.

Specification

• Description: This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
• Source: HEK293
• Species: Human
• Tag: His
• Form: Powder
• Bio-activity: Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10 ng/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression.
• Protein length: Arg31-Ala263
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 92% (by SDS-PAGE)
• Applications: FuncSt, SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: PBS (pH 7.4)
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/ml in sterile distilled water.

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• Protein Refseq: NP_001033722
• MIM: 609595
• UniProt ID: Q2MKA7