Active Recombinant Human RNF43 protein, Fc-tagged

• Cat.No.: RNF43-0655H
• Product Overview: Recombinant Human RNF43 protein(Gln44-Tyr197), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human R-Spondin 1, His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 2-20 ng/mL. Immobilized Human R-Spondin 3 (22-146), His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 0.3-2 ng/mL. Immobilized Human R-Spondin 1 (21-146), His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 0.01-0.156 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln44-Tyr197
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 44-197 a.a.

Gene Information

• Gene Name: RNF43 ring finger protein 43 [ Homo sapiens ]
• Official Symbol: RNF43
• Synonyms: URCC; RNF124
• Gene ID: 54894
• mRNA Refseq: NM_017763.4
• Protein Refseq: NP_060233.3
• MIM: 612482
• UniProt ID: Q68DV7