Active Recombinant Human RHOA protein, GST-tagged
Cat.No. : | RHOA-34H |
Product Overview : | Recombinant Human RHOA fused with GST tag in the amino-terminus was expressed in E. coli. |
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Species : | Human |
Source : | E.coli |
Tag : | GST |
Description : | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overex |
Form : | The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. |
Bio-activity : | The biological activity of RhoA proteins was monitored by measuring the ability of the protein to hydrolyze GTP in the presence and absence of a GTPase-activating protein. |
Molecular Mass : | The recombinant protein is 52 kDa, consisting of the 24 kDa RhoA protein plus a 28 kDa GST tag in the amino-terminus. |
Purity : | 90% |
Applications : | RhoA biochemistry; RhoA GTPase assays; RhoA nucleotide exchange assays; RhoA binding studies. |
Gene Name | RHOA ras homolog family member A [ Homo sapiens ] |
Official Symbol | RHOA |
Synonyms | RHOA; ras homolog family member A; ARH12, ARHA, ras homolog gene family, member A; transforming protein RhoA; Rho12; RhoA; RHOH12; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A; ARHA; ARH12; RHO12; |
Gene ID | 387 |
mRNA Refseq | NM_001664 |
Protein Refseq | NP_001655 |
MIM | 165390 |
UniProt ID | P61586 |
Chromosome Location | 3p21.3 |
Pathway | Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; |
Function | GTP binding; GTPase activity; myosin binding; nucleotide binding; protein binding; |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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