Active Recombinant Human Ret Proto-Oncogene, GST-His-tagged

• Cat.No.: RET-5476H
• Product Overview: Recombinant humanRET contains amino acids H658-D1110 (as in GenBank entry NM_000323) and isN-terminally fused to GST-His6-Thrombin cleavage site.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST&His
• Protein Length: 658-1110 a.a.
• Description: The RETproto-oncogene encodes a receptor tyrosine kinase for members of the glialcell line-derived neurotrophic factor family of extracellular signallingmolecules. RET loss of function mutations are associated with the developmentof Hirschsprung" s disease, while gain of function mutations are associatedwith the development of various types of human cancer.
• Form: Liquid in 50 mMTris-HCl, pH 8.0 + 100 mM NaCl + 5 mM DTT + 15 mM reduced glutathione + 20%glycerol.
• MolecularWeight: 84.322 kDa
• Specific Activity: 24 pmol/μg x min.Assay conditions: 60 mM HEPES-NaOH, pH 7.5 + 3 mM MgCl2 + 3 mMMnCl2 + 3 μM Na-orthovanadate + 1.2 mM DTT + 2.5 μg / 50 μl PEG20.000+ ATP (variable) + 2 μg / 50 μl Poly(Glu:Tyr)4:1 substrate + 200ng / 50 μl recombinant RET.
• Concentration: 0.131 mg/ml
• Storage: Store in workingaliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: RET
• Pathways: Endocytosis;Pathways in cancer; SIDS Susceptibility Pathways; Signaling events regulatedby Ret tyrosine kinase; Thyroid cancer

Gene Information

• Gene Name: RET ret proto-oncogene [ Homosapiens ]
• Synonyms: RET; retproto-oncogene; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16;RET-ELE1; proto-oncogenetyrosine-protein kinase receptor Ret; OTTHUMP00000019479; OTTHUMP00000216967;proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence; cadherinfamily member 12; hydroxyaryl-protein kinase; cadherin-related family member16; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroidcarcinoma 1, Hirschsprung disease); EC 2.7.10.1; Proto-oncogene tyrosine-protein kinasereceptor Ret; Cadherinfamily member 12; Proto-oncogene c-Ret
• Gene ID: 5979
• mRNA Refseq: NM_020630
• Protein Refseq: NP_065681
• MIM: 164761
• UniProt ID: P07949
• Chromosome Location: 10q11.2
• Function: ATP binding; calciumion binding; nucleotide binding; protein binding; protein tyrosine kinaseactivity; receptor activity; transmembrane receptor protein tyrosine kinaseactivity