Active Recombinant Human PVRL2 protein, mFc-Avi-tagged, Biotinylated

• Cat.No.: PVRL2-0571H
• Product Overview: Biotinylated Recombinant Human PVRL2 protein(Gln32-Leu360), fused with mFc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&mFc
• Protein Length: 32-360 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human PVRIG, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag with a linear range of 0.6-10 ng/mL. Immobilized Human DNAM-1, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag with a linear range of 0.01-0.625 μg/mL. Immobilized Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human PVRIG, His Tag with a linear range of 1-20 ng/mL.
• Molecular Mass: This protein carries a mouse IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 64.1 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln32-Leu360
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL2 poliovirus receptor-related 2 (herpesvirus entry mediator B) [ Homo sapiens ]
• Official Symbol: PVRL2
• Synonyms: PVRL2; poliovirus receptor-related 2 (herpesvirus entry mediator B); HVEB; poliovirus receptor-related protein 2; CD112; PRR2; PVRR2; nectin-2; poliovirus receptor-like 2; herpesvirus entry protein B
• Gene ID: 5819
• mRNA Refseq: NM_001042724
• Protein Refseq: NP_001036189
• MIM: 600798
• UniProt ID: Q92692