Active Recombinant Human PVRL2 protein, His-Avi-tagged, Biotinylated

• Cat.No.: PVRL2-612H
• Product Overview: Biotinylated Recombinant Human PVRL2 protein(Gln 32 - Leu 360), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln 32 - Leu 360
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Biotinylated Human Nectin-2, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human PVRIG, Fc Tag with a linear range of 0.6-10 ng/mL. Immobilized Biotinylated Human Nectin-2, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human DNAM-1, Fc Tag with a linear range of 0.039-0.625 μg/mL. Immobilized Biotinylated Human Nectin-2, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Nectin-3, Fc Tag with a linear range of 0.039-0.625 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 39.1 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL2 poliovirus receptor-related 2 (herpesvirus entry mediator B) [ Homo sapiens ]
• Official Symbol: PVRL2
• Synonyms: PVRL2; poliovirus receptor-related 2 (herpesvirus entry mediator B); HVEB; poliovirus receptor-related protein 2; CD112; PRR2; PVRR2; nectin-2; poliovirus receptor-like 2; herpesvirus entry protein B
• Gene ID: 5819
• mRNA Refseq: NM_001042724
• Protein Refseq: NP_001036189
• MIM: 600798
• UniProt ID: Q92692