Active Recombinant Human PTPN12 protein, His&GST-tagged

• Cat.No.: PTPN12-467H
• Product Overview: Recombinant Human PTPN12 protein(AAA36529.1)(Met1-Gln355), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Met1-Gln355
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate.The specific activity is > 15 μmoles/min/mg.
• Molecular Mass: The recombinant human PTPN12 chimera consists of 592 amino acids and has a calculated molecular mass of 69.4 kDa. The recombinant protein migrates approximately 64 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PTPN12 protein tyrosine phosphatase, non-receptor type 12 [ Homo sapiens ]
• Official Symbol: PTPN12
• Synonyms: PTPN12; protein tyrosine phosphatase, non-receptor type 12; tyrosine-protein phosphatase non-receptor type 12; PTP PEST; PTPG1; protein-tyrosine phosphatase G1; PTP-PEST
• Gene ID: 5782
• mRNA Refseq: NM_001131008
• Protein Refseq: NP_001124480
• MIM: 600079
• UniProt ID: Q05209