Active Recombinant Human PRSS8 protein(Ala30-Arg322), His-tagged

• Cat.No.: PRSS8-315H
• Product Overview: Recombinant Human Prss8 (NP_002764.1) (Ala 30-Arg 322) was expressed in HEK293, fused with a signal peptide at the N-terminus and a polyhistidine-tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ala30-Arg322
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC. The specific activity is >10 pmoles/min/μg.
• Molecular Mass: The mature recombinant human Prss8 consists of 304 amino acids and predicts a molecular mass of 32.8 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhPrss8 is approximately 40 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PRSS8 protease, serine, 8 [ Homo sapiens ]
• Official Symbol: PRSS8
• Synonyms: PRSS8; protease, serine, 8; prostasin; serine protease 8; channel-activating protease 1; CAP1; PROSTASIN
• Gene ID: 5652
• mRNA Refseq: NM_002773
• Protein Refseq: NP_002764
• MIM: 600823
• UniProt ID: Q16651