Active Recombinant Human PRSS27 protein, His-tagged

• Cat.No.: PRSS27-1176H
• Product Overview: Recombinant Human PRSS27 protein(Q9BQR3)(Met1-Lys290), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Lys290
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, NcarbobenzyloxyGlyArgThioBenzyl ester (ZGR-SBzl), in the presence of 5,5’Dithiobis (2-nitrobenzoic acid) (DTNB).The specific activity is > 2000 pmols/min/μg.
• Molecular Mass: The recombinant human PRSS27 consists of 279 amino acids and has a predicted molecular mass of 30.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh PRSS27 is approximately 42 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PRSS27 protease, serine 27 [ Homo sapiens ]
• Official Symbol: PRSS27
• Synonyms: PRSS27; protease, serine 27; serine protease 27; CAPH2; marapsin; MPN; pancreasin; channel-activating protease 2
• Gene ID: 83886
• mRNA Refseq: NM_031948
• Protein Refseq: NP_114154
• MIM: 608018
• UniProt ID: Q9BQR3