Active Recombinant Human PRSS2 Protein, His-tagged

• Cat.No.: PRSS2-30H
• Product Overview: Recombinant Human PRSS2 Protein (Ala16-Ser247) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: NS0
• Tag: His
• Protein Length: 16-247 a.a.
• Description: This gene belongs to the trypsin family of serine proteases and encodes anionic trypsinogen. It is part of a cluster of trypsinogen genes that are located within the T cell receptor beta locus. Enzymes of this family cleave peptide bonds that follow lysine or arginine residues. This protein is found at high levels in pancreatic juice and its upregulation is a characteristic feature of pancreatitis. This protein has also been found to activate pro-urokinase in ovarian tumors, suggesting a function in tumor invasion. In addition, this enzyme is able to cleave across the type II collagen triple helix in rheumatoid arthritis synovitis tissue, potentially participating in the degradation of type II collagen-rich cartilage matrix. Alternative splicing results in multiple transcript variants.
• Form: Pro form
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 8,000 pmol/min/μg
• Molecular Mass: 34 kDa, reducing conditions
• N-terminal Sequence Analysis: Ala16
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 95 % by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in HCl and NaCl.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: PRSS2 serine protease 2 [ Homo sapiens (human) ]
• Official Symbol: PRSS2
• Synonyms: PRSS2; serine protease 2; TRY2; TRY8; TRYP2; trypsin-2; anionic trypsinogen; protease serine 2 preproprotein; protease, serine 2; protease, serine, 2 (trypsin 2); trypsin II; trypsinogen 2; EC 3.4.21.4
• Gene ID: 5645
• mRNA Refseq: NM_002770
• Protein Refseq: NP_002761
• MIM: 601564
• UniProt ID: P07478