Active Recombinant Human PROM1 protein, His-Avi-tagged, Biotinylated(Nanodisc)

• Cat.No.: PROM1-3972H
• Product Overview: Biotinylated Recombinant Human PROM1 protein(O43490-1)(Gly 20 - His 865) is expressed from HEK293 with His tag and Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: Gly 20 - His 865
• Form: Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Biotinylated Human CD133 Protein, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD133 Antibody, Human IgG1 with a linear range of 0.6-78 ng/mL.
• Molecular Mass: The protein has a calculated MW of 98.9 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >80% as determined by SDS-PAGE.
• Storage: This product is stable after storage at:
  The product MUST be stored at -70°C or lower upon receipt;
  -70°C for 3 months under sterile conditions.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PROM1 prominin 1 [ Homo sapiens ]
• Official Symbol: PROM1
• Synonyms: PROM1; prominin 1; macular dystrophy, retinal 2 , MCDR2, prominin (mouse) like 1 , PROML1, Stargardt disease 4 (autosomal dominant) , STGD4; prominin-1; AC133; CD133; RP41; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen; MCDR2; STGD4; CORD12; PROML1; MSTP061
• Gene ID: 8842
• mRNA Refseq: NM_001145847
• Protein Refseq: NP_001139319
• MIM: 604365
• UniProt ID: O43490