Active Recombinant Human PPP3CA&PPP3R1

• Cat.No.: PPP3CA&PPP3R1-626H
• Product Overview: Recombinant Human PPP3CA&PPP3R1 (Accession # NP_000935 (Calcineurin A) NP_000936 (Calcineurin B) was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Non
• Predicted N Terminal: Asp15 (Calcineurin A) & Asp17 (Calcineurin B)
• Form: Supplied as a 0.2 µm filtered solution in HEPES, CaCl2, MgCl2, DTT and Glycerol.
• Bio-activity: Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE.The specific activity is >900 nmol/min/mg, under the described conditions.
• Molecular Mass: Recombinant Human PPP3CA&PPP3R1 has a calculated MW of 57 kDa (Calcineurin A), 18 kDa (Calcineurin B). In SDS-PAGE migrates as 43-65 kDa & 15-20 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme [ Homo sapiens ]
• Official Symbol: PPP3CA
• Synonyms: PPP3CA; protein phosphatase 3, catalytic subunit, alpha isozyme; CALN, CALNA, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform , protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha); serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CNA1; PPP2B; protein phosphatase 2B; catalytic subunit; alpha isoform; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; CALN; CCN1; CALNA; CALNA1
• Gene ID: 5530
• mRNA Refseq: NM_000944
• Protein Refseq: NP_000935
• MIM: 114105
• UniProt ID: Q08209
• Chromosome Location: 4q24