Active Recombinant Human PON1 Protein, His-tagged

• Cat.No.: PON1-01H
• Product Overview: Recombinant human PON1 (16-355) protein with a His-tag was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 16-355
• Description: This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7.
• Form: Liquid
• Bio-activity: Specific activity is > 2,500 pmol/min/µg, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37 centigrade.
• Molecular Mass: 39 kDa
• Endotoxin: < 1 EU per 1 µg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles
• Concentration: 0.25 mg/ml
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Gene Information

• Gene Name: PON1 paraoxonase 1 [ Homo sapiens (human) ]
• Official Symbol: PON1
• Synonyms: PON1; paraoxonase 1; ESA; PON; MVCD5; serum paraoxonase/arylesterase 1; A-esterase 1; K-45; PON 1; aromatic esterase 1; arylesterase 1; arylesterase B-type; esterase A; paraoxonase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1
• Gene ID: 5444
• mRNA Refseq: NM_000446
• Protein Refseq: NP_000437
• MIM: 168820
• UniProt ID: P27169