Active Recombinant Human POMGNT1, His-tagged

• Cat.No.: POMGNT1-289H
• Product Overview: Recombinant Human POMGNT1 (Accession # AAH01471) Ser66-Thr660, fused with an N-terminal human CD33 signal sequence and a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• ProteinLength: 66-660 a.a.
• Predicted N Terminal: Ser66
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to transfer N-Acetyl-D-glucosamine from UDP-GlcNAc to Methyl-alpha -D-mannopyronoside.The specific activity is >500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human POMGNT1, His-tagged has a calculated MW of 68 kDa. In SDS-PAGE migrates as 65-80 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: POMGNT1 protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase [ Homo sapiens ]
• Official Symbol: POMGNT1
• Synonyms: POMGNT1; protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase; protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1; FLJ20277; MGAT1.2; protein O mannose beta 1; 2 N acetylglucosaminyltransferase; UDP-GlcNAc:alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2; MEB; GNTI.2; MDDGA3; MDDGB3; MDDGC3; GnT I.2; gnT-I.2; DKFZp761B182
• Gene ID: 55624
• mRNA Refseq: NM_001243766
• Protein Refseq: NP_001230695
• MIM: 606822
• UniProt ID: Q8WZA1
• Chromosome Location: 1p34.1