Active Recombinant Human PLK1 protein, His-tagged

• Cat.No.: PLK1-785H
• Product Overview: Recombinant Human PLK1 protein(NP_005021.2)(Met1-Ser603), fused with His tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Ser603
• Form: Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.4, 0.5mM EDTA, 0.5mM EGTA, 0.5mM PMSF, 25% glycerolPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be ＞3 nmol/min/mg using casein as substrate.
• Molecular Mass: The recombinant human PLK1 consists of 621 amino acids and predicts a molecular mass of 70.5 kDa. It migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PLK1 polo-like kinase 1 [ Homo sapiens ]
• Official Symbol: PLK1
• Synonyms: PLK1; polo-like kinase 1; PLK, polo like kinase (Drosophila); serine/threonine-protein kinase PLK1; PLK-1; polo like kinase; polo (Drosophia)-like kinase; serine/threonine-protein kinase 13; cell cycle regulated protein kinase; PLK; STPK13
• Gene ID: 5347
• mRNA Refseq: NM_005030
• Protein Refseq: NP_005021
• MIM: 602098
• UniProt ID: P53350