Active Recombinant Human PIN4 protein, His-tagged

• Cat.No.: PIN4-974H
• Product Overview: Recombinant Human PIN4(1-156aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF
• Bio-activity: Specific activity is >300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
• Molecular Mass: 18.8 kDa (176aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSHEKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRKMPMAGLLKGL VRQLERFSVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHIL
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Protein length: 1-156 a.a.

Gene Information

• Gene Name: PIN4 protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin) [ Homo sapiens ]
• Official Symbol: PIN4
• Synonyms: PIN4; protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin); protein (peptidyl prolyl cis/trans isomerase) NIMA interacting, 4 (parvulin); peptidyl-prolyl cis-trans isomerase NIMA-interacting 4; EPVH; PAR14; PAR17; hEPVH; hPar14; hPar17; PPIase PIN4; parvulin-14; parvulin-17; rotamase PIN4; eukaryotic parvulin homolog; peptidyl-prolyl cis-trans isomerase Pin4; peptidyl-prolyl cis/trans isomerase EPVH; MGC138486
• Gene ID: 5303
• mRNA Refseq: NM_001170747
• Protein Refseq: NP_001164218
• MIM: 300252
• UniProt ID: Q9Y237
• Chromosome Location: Xq13.1
• Function: DNA binding; bent DNA binding; double-stranded DNA binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity