Active Recombinant Human PI4KB, GST-tagged

• Cat.No.: PI4KB-1077H
• Product Overview: Full-length recombinant human PI4KB was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PI4KB is a member of the phosphatidylinositol 4-kinase family (PI4K) and is the first committed step in the production of the second messenger inositol-1,4,5-trisphosphate (PIP3). PI4KB regulates the golgi disintegration/reorganization during mitosis possibly via the phosphorylation of phosphatidylinositol. PI4KB is a type III enzyme that is sensitive to wortmannin and is responsible for regulating the synthesis of agonist-sensitive pools of polyphosphoinositides. PI4KB is critical for glucose-induced insulin secretion due to its capacity to regulate the release of secretory granules. PI4KB inhibits the insulin-stimulated translocation of glucose transporter-4 in mouse adipocytes through its interaction with neuronal calcium sensor-1.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 37 nmol/min/mg
• Molecular Mass: ~120 kDa
• Protein length: Full Length
• Purity: >90%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PI4KB phosphatidylinositol 4-kinase, catalytic, beta [ Homo sapiens ]
• Official Symbol: PI4KB
• Synonyms: PI4KB; phosphatidylinositol 4-kinase, catalytic, beta; PIK4CB; phosphatidylinositol 4-kinase beta; PI4K BETA; pi4K92; PtdIns 4-kinase beta; type III phosphatidylinositol 4-kinase beta; phosphatidylinositol 4-kinase, wortmannin-sensitive; NPIK; PI4K92; PI4KBETA; PI4K-BETA; PI4KIIIBETA; FLJ30129; DKFZp686O1820
• Gene ID: 5298
• mRNA Refseq: NM_001198773
• Protein Refseq: NP_001185702
• MIM: 602758
• UniProt ID: Q9UBF8
• Chromosome Location: 1q21
• Pathway: 3-phosphoinositide biosynthesis; D-myo-inositol (1,4,5)-trisphosphate biosynthesis; Inositol phosphate metabolism
• Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; protein binding