Active Recombinant Human PI4KA protein, GST-tagged

• Cat.No.: PI4KA-161H
• Product Overview: Recombinant Human PI4KA fused with an N-terminal GST tag was expressed in Insect cells.

Specification

• Description: PI4KA encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by PI4KA gene is a type III enzyme that is not inhibited by adenosine. PI4KA is highly expressed in placenta and brain. PI4KA is associated with the membranes of Golgi vesicles and vacuoles.
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 70 nmol/min/mg
• Molecular Mass: ~120 kDa
• Purity: >70%
• Applications: Kinase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05μg/μl
• Publications:
  PI-273, a substrate-competitive, specific small molecule inhibitor of PI4KIIα, inhibits the growth of breast cancer cells (2017)
  PI-273, a substrate-competitive, specific small molecule inhibitor of PI4KIIα, inhibits the growth of breast cancer cells (2017)

Gene Information

• Gene Name: PI4KA phosphatidylinositol 4-kinase, catalytic, alpha [ Homo sapiens ]
• Official Symbol: PI4KA
• Synonyms: PI4KA; phosphatidylinositol 4-kinase, catalytic, alpha; PIK4CA; phosphatidylinositol 4-kinase alpha; PI4K ALPHA; pi4K230; PI4-kinase alpha; ptdIns-4-kinase alpha; phosphatidylinositol 4-kinase 230; phosphatidylinositol 4-kinase, type III, alpha; PI4K-ALPHA; FLJ16556
• Gene ID: 5297
• mRNA Refseq: NM_002650
• Protein Refseq: NP_002641
• MIM: 600286
• UniProt ID: P42356
• Chromosome Location: 22q11.21
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Inositol phosphate metabolism, PI=>
• Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; nucleotide binding; phosphatidylinositol 3-kinase activity; protein binding