Active Recombinant Human PI4K2B, GST-tagged

• Cat.No.: PI4K2B-1451H
• Product Overview: Full-length recombinant human PI4K2B was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PI4K2B is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are immediate precursor of several important signaling and scaffolding molecules. PI4K2B is primarily a cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 4,5-bisphosphate syntheses. PI4K2B uses phosphatidylinositol as their primary substrate and has no activity on phosphatidylinositol monophosphates. PI4K2B is a restricted minor histocompatibility antigen in patients that had been successfully treated with donor lymphocyte infusions for relapsed chronic myeloid leukemia after allogeneic stem cell transplantation.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 218 nmol/min/mg
• Molecular Mass: ~84 kDa
• Protein length: Full length
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PI4K2B phosphatidylinositol 4-kinase type 2 beta [ Homo sapiens ]
• Official Symbol: PI4K2B
• Synonyms: PI4K2B; phosphatidylinositol 4-kinase type 2 beta; phosphatidylinositol 4-kinase type 2-beta; FLJ11105; PI4KIIB; PIK42B; PI4KII-BETA; phosphatidylinositol 4-kinase type II-beta; phosphatidylinositol 4-kinase type-II beta
• Gene ID: 55300
• mRNA Refseq: NM_018323
• Protein Refseq: NP_060793
• MIM: 612101
• UniProt ID: Q8TCG2
• Chromosome Location: 4p15.31
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Inositol phosphate metabolism, PI=>
• Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; kinase activity; nucleotide binding; transferase activity