Active Recombinant Human PDK4 Protein, GST-tagged

• Cat.No.: PDK4-1073H
• Product Overview: Recombinant full-length human PDHK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Insect cells
• Tag: GST
• Description: This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
• Bio-activity: 20 nmol/min/mg
• Molecular Mass: ~71 kDa
• Purity: >90%
• Stability: One year at -70 centigrade from the date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Shipping: Dry ice.

Gene Information

• Gene Name: PDK4 pyruvate dehydrogenase kinase 4 [ Homo sapiens (human) ]
• Official Symbol: PDK4
• Synonyms: PDK4; pyruvate dehydrogenase kinase 4; pyruvate dehydrogenase kinase, isozyme 4; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial; EC 2.7.11.2
• Gene ID: 5166
• mRNA Refseq: NM_002612
• Protein Refseq: NP_002603
• MIM: 602527
• UniProt ID: Q16654