Active Recombinant Human PDIA6 protein, His-tagged

• Cat.No.: PDIA6-22H
• Product Overview: Recombinant human PDIA6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: PDIA6 is a member of the protein disulfide isomerase (PDI). PDI is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. PDIA6 function as a chaperone that inhibits aggregation of misfolded proteins. It plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing, 50mM NaCl, 2mM DTT and 10% glycerol
• Bio-activity: Specific activity >20 A650/cm/min/mg. Enzymatic activitywas confirmed by measuring the aggregation of insulin in the presence of DTT.
• Molecular Mass: 48.5 kDa (442aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MLYSSSDDVI ELTPSNFNRE VIQSDSLWLV EFYAPWCGHC QRLTPEWKKA ATALKDVVKV GAVDADKHHS LGGQYGVQGF PTIKIFGSNK NRPEDYQGGR TGEAIVDAAL SALRQLVKDR LGGRSGGYSS GKQGRSDSSS KKDVIELTDD SFDKNVLDSE DVWMVEFYAP WCGHCKNLEP EWAAAASEVK EQTKGKVKLA AVDATVNQVL ASRYGIRGFP TIKIFQKGES PVDYDGGRTR SDIVSRALDLFSDNAPPPEL LEIINEDIAK RTCEEHQLCV VAVLPHILDT GAAGRNSYLE VLLKLADKYK KKMWGWLWTE AGAQSELETA LGIGGFGYPA MAAINARKMK FALLKGSFSE QGINEFLREL SFGRGSTAPV GGGAFPTIVE REPWDGRDGE LPVEDDIDLS DVELDDLGKD EL
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PDIA6 protein disulfide isomerase family A, member 6 [ Homo sapiens ]
• Official Symbol: PDIA6
• Synonyms: PDIA6; protein disulfide isomerase family A, member 6; protein disulfide isomerase associated 6 , thioredoxin domain containing 7 (protein disulfide isomerase) , TXNDC7; protein disulfide-isomerase A6; ERp5; P5; protein disulfide isomerase related protein; ER protein 5; protein disulfide isomerase P5; endoplasmic reticulum protein 5; thioredoxin domain-containing protein 7; protein disulfide isomerase-associated 6; protein disulfide isomerase-related protein; thioredoxin domain containing 7 (protein disulfide isomerase); ERP5; TXNDC7
• Gene ID: 10130
• mRNA Refseq: NM_005742
• Protein Refseq: NP_005733
• MIM: 611099
• UniProt ID: Q15084
• Chromosome Location: 2p25.1
• Pathway: Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem; Unfolded Protein Response, organism-specific biosystem
• Function: electron carrier activity; isomerase activity; protein disulfide isomerase activity; protein disulfide oxidoreductase activity