Active Recombinant Human PDGFB

• Cat.No.: PDGFB-146H
• Product Overview: Recombinant Human platelet-derived growth factor was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: The platelet-derived growth factor (PDGF) family consists of four different polypeptide chains; PDGF-A, PDGF-B, PDGF-C and PDGF-D, sharing approximately 25% sequence homology. The biological effects of PDGF-B are exerted via two receptor tyrosine kinases, PDGFRA and PDGFRB. PDGF-B is expressed predominantly in platelets and the heart, but has been detected in the majority of human organs. PDGF-B is a potent mitogen for mesenchymal cells and plays a critical role in wound healing and tumor development. PDGF-B is an important growth factor contributing to angiogenesis and possibly lymphatic metastasis due to lymphangiogenesis. Additionally, PDGF-B plays a crucial role in wound healing. Studies have also suggested that PDGF-B is involved in the CNS including having a neuroprotective or neurotrophic effect in the developing or adult CNS, promoting angiogenic responses to injury in the CNS and remyelination..
• Molecular Mass: Under reducing conditions PDGF-B migrates as a broad band between 15.2 and 16.1 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified PDGF-B polypeptide that has a predicted monomeric molecular mass of 12.29 kDa.
• PI: PDGF-B has a predicted pI of 9.38.
• % Carbohydrate: purified PDGF-B consists of 30-45% carbohydrate by weight.
• Glycosylation: PDGF-B contains N-linked oligosaccharides and a predicted O-linked Mannose.
• Purity: >95%, as determined by SDS-PAGE, visualized by Coomassie Brilliant Blue.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C.
• Activity: The ED₅₀of PDGF-B is typically 14-25 ng/mL as measured in a cell proliferation assay using the murine Balb/3T3 fibroblast cell line.
• Theoretical Sequence: SLGSLTI AEPAMIAECK TRTEVFEISRRLI DRTNANFLVWPPC VEVQRCSGCCNNR NV QCRP TQVQLRPV QVRKI EIVRK KPIFKK ATVTLEDHLACKCETVAAARPV.

Gene Information

• Gene Name: PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) [ Homo sapiens ]
• Synonyms: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; SSV; PDGF2; c-sis; FLJ12858; PDGFB; platelet-derived growth factor beta; becaplermin; PDGF, B chain; platelet-derived growth factor 2; platelet-derived growth factor, B chain;Platelet-derived growth factor, beta polypeptide (oncogene SIS); oncogene SIS; Becaplermin; PDGF-2; PDGF subunit B; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor B chain
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• MIM: 190040
• UniProt ID: P01127
• Chromosome Location: 22q13.1
• Pathway: Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Hemostasis; Signaling by PDGF
• Function: cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity